Translational_Unit

Part:BBa_K3803013:Design

Designed by: Shiyu Yu   Group: iGEM21_Jiangnan_China   (2021-09-02)


XR translational unit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 805
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 805
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 805
    Illegal BglII site found at 1265
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 805
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 805
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon of XR from Scheffersomyces stipitis had been optimized for better expression in S. cerevisiae.


Source

The promoter and terminator is from S. cerevisiae, and the coding gene of XR is from Scheffersomyces stipitis.

References